Interlaboratory Study of DNA Extraction from Multiple Ground Samples, Multiplex Real-Time PCR, and Multiplex Qualitative PCR for Individual Kernel Detection System of Genetically Modified Maize
نویسندگان
چکیده
منابع مشابه
Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis.
Multiplex PCR procedures were developed for simultaneously detecting multiple target sequences in genetically modified (GM) soybean (Roundup Ready), maize (event 176, Bt11, Mon810, T14/25), and canola (GT73, HCN92/28, MS8/RF3, Oxy 235). Internal control targets (invertase gene in corn, lectin and beta-actin genes in soybean, and cruciferin gene in canola) were included as appropriate to assess ...
متن کاملReal-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
متن کاملDetection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR
Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer’s right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following extraction of genomic DNA ...
متن کاملDetection of genetically modified crops by combination of multiplex PCR and low-density DNA microarray.
OBJECTIVE To develop a technique for simultaneous detection of various target genes in Roundup Ready soybean by combining multiplex PCR and low-density DNA microarray. METHODS Two sets of the multiplex PCR system were used to amplify the target genes in genetically modified (GM) soybean. Seventeen capture probes (PCR products) and 17 pairs of corresponding primers were designed according to t...
متن کاملDetection of genetically modified maize in processed foods sold commercially in Iran by qualitative PCR
Detection of genetically modified organisms (GMOs) in food is an important issue for all the subjects involved in food control and customer’s right. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. In this study, following extraction of genomic DNA ...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2011
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/94.5.1540